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. 2022 Oct 3;20(12):2418–2429. doi: 10.1111/pbi.13924

Figure 2.

Figure 2

Detection of synthetically produced herbicide resistance mutations in rice. (a) Partial sequence alignment of the evolved gene SF3B1 in rice lines SGR3 and SGR5 and in the wild type (WT). SGR3 harbours a deletion of residue K1050. SGR5 differs from the WT by a H1048Q substitution and a deletion of residue K1049. (b) Bio‐SCAN specifically detects both synthetically evolved sequences in the SGR3 and SGR5 lines with their respective sgRNAs. Neither SGR3‐sgRNA nor SGR5‐sgRNA detected the WT sequence for the SF3B1 gene. (c) Partial sequence alignment of ALS in the mutant and the wild type. mALS differs from the WT protein by a W548L substitution and a silent G‐to‐A substitution that does not affect the E549 residue. (d) Bio‐SCAN specifically detects the mALS sequence. C, control line; T, test line. The arrowhead indicates the location of the expected band at the T line.