Figure 5.

BSMV‐sgRNA‐based gene editing of the spring and winter cultivars. Introgression of the highly expressed Cas9 allele from the high‐Cas9 707 line (Figure S1) into (a) CIMMYT line 3 613 474 (spring wheat) and (b) Kansas line KS080093K‐18 (winter wheat). The BC2F3 and BC1F2 lines derived from the crosses with 3 613 474 and KS080093K‐18, respectively, were used for BSMV inoculation. (c) The somatic editing efficiency was estimated (1) for BSMV‐QT1 and BSMV‐GW7T6 constructs used to inoculate lines derived from 3 613 474 and (2) for BSMV‐GW7T6 used to inoculate lines derived from KS080093K‐18. The editing efficiency was evaluated in the 4th leaf of inoculated plants. The data from all three genomes are combined to calculate the editing efficiency. (d) The summary of the M₁ generation mutants obtained by the BSMV‐sgRNA‐based editing of wheat cultivars expressing the introgressed high‐Cas9 allele.