. 2022 Nov 18;11:e82094. doi: 10.7554/eLife.82094

Video 6. Rab5-positive early endosomes form a cloud that follows the centrosome into phagocytic branches.

Right: representative microglia (magenta; Tg(csf1ra:GAL4-VP16; UAS:lyn-tagRFPt)), expressing the Rab5 endosome (cyan; UAS:mNeonGreen-Rab5a) and centrosome (yellow; UAS:miRFP670-centrin4) reporters. Left: image segmentation showing tracking of the centrosome (red dot) and a binary threshold view of the Rab5 signal (black pixels) to show cloud dynamics. Images were captured every 15 s for 30 min using single-plane illumination microscopy (SPIM). Timescale is hh:mm:ss.