. 2022 Nov 18;11:e82094. doi: 10.7554/eLife.82094

Video 8. Microglia centrosome is retained within the cell soma after phorbol 12-myristate 13-acetate (PMA) and pan-inhibitor of PLC (PLCi) treatment.

Representative microglia treated with DMSO, PMA, and PLCi (grey; Tg(csf1ra:GAL4-VP16; UAS:lyn-tagRFPt)) expressing the centrosome reporter (cyan; Tg(UAS:miRFP670-cetn4)). Images were captured every 30 s for 1 hr using single-plane illumination microscopy (SPIM). Original images were processed with a Gaussian Blur filter with sigma(radius) = 1. Timescale is mm:ss.