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. 2022 Nov 18;11:e82094. doi: 10.7554/eLife.82094
Vector Primer sequence (5′–3′)
Lamp1-mGFP pENTR/D-TOPO cloning FW CACCTTCAGGGACATGGCGGCC
REV TTACTTGTACAGCTCGTCCATGCCG
pC2_EB3-GFP
Gibson Assembly P1
FW CAGGCTGGATGGCCGTCAATGTGTAC
REV GGTGGCGACCGGTGGATCCAGGTACTCGTCCTGGTCTTC
pmScarlet-i_C1
Gibson Assembly P2
FW CTGGATCCACCGGTCGCCACCATGGTGAGCAAGGGCGAG
REV TTACTTGTACAGCTCGTCCATGC
pME_mNeonGreen
Gibson Assembly P3
FW GACGAGCTGTACAAGTAAGACCCAGCTTTCTTGTAC
REV CGGCCATCCAGCCTGCTTTTTTGTAC
p3E_cetn4
Gateway BP cloning
FW GGGGACAGCTTTCTTGTACAAAGTGGGGatggcgtccggcttcaggaaaag
REV GGGGACAACTTTGTATAATAAAGTTGGgtacagattggttttcttcataat
pME_miRFP670
Gateway BP cloning
FW GGGGACAAGTTTGTACAAAAAAGCAGGCTGGATGGTAGCAGGT
CATGCCTCTGgcagccc
REV GGGGACCACTTTGTACAAGAAAGCTGGGTCGCTCTCAAGCGCG
GTGAtccgc