Vector | Primer sequence (5′–3′) |
Lamp1-mGFP pENTR/D-TOPO cloning |
FW CACCTTCAGGGACATGGCGGCC
REV TTACTTGTACAGCTCGTCCATGCCG |
pC2_EB3-GFP Gibson Assembly P1 |
FW CAGGCTGGATGGCCGTCAATGTGTAC
REV GGTGGCGACCGGTGGATCCAGGTACTCGTCCTGGTCTTC |
pmScarlet-i_C1 Gibson Assembly P2 |
FW CTGGATCCACCGGTCGCCACCATGGTGAGCAAGGGCGAG
REV TTACTTGTACAGCTCGTCCATGC |
pME_mNeonGreen Gibson Assembly P3 |
FW GACGAGCTGTACAAGTAAGACCCAGCTTTCTTGTAC
REV CGGCCATCCAGCCTGCTTTTTTGTAC |
p3E_cetn4 Gateway BP cloning |
FW GGGGACAGCTTTCTTGTACAAAGTGGGGatggcgtccggcttcaggaaaag
REV GGGGACAACTTTGTATAATAAAGTTGGgtacagattggttttcttcataat |
pME_miRFP670 Gateway BP cloning |
FW GGGGACAAGTTTGTACAAAAAAGCAGGCTGGATGGTAGCAGGT
CATGCCTCTGgcagccc REV GGGGACCACTTTGTACAAGAAAGCTGGGTCGCTCTCAAGCGCG GTGAtccgc |