Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HCT116 (adult colorectal carcinoma) | ECACC | Cat# 91091005, RRID:CVCL_0291 | |
| Cell line (Homo sapiens) | SW480 (adult colorectal carcinoma) | ECACC | Cat# 87092801, RRID:CVCL_0546 | |
| Transfected construct (Homo sapiens) | Human-ESRP1 shRNA | Horizon | Cat# V3THS_400802 | Lentiviral construct to transfect express the shRNA |
| Antibody | Anti-human ZEB1 (rabbit monoclonal) | Cell Signaling | Cat# 3396, RRID:AB_1904164 | WB (1.1000) |
| Antibody | Anti-human ESRP1 (rabbit polyclonal) | Thermo Fisher | Cat# PA5-11520, RRID:AB__2899836 | WB (1.1000) |
| Antibody | Anti-human CD44s (mouse monoclonal) | Thermo Fisher | Cat# MA5-13890, RRID:AB_10986810 | WB (1.100) |
| Antibody | Anti-human CD44v6 (mouse monoclonal) | Abcam | Cat# ab78960, RRID:AB_1603730 | WB (1.1000) |
| Antibody | Anti-human NUMB (rabbit monoclonal) | Cell Signaling | Cat# 2756, RRID:AB_2534177 | WB (1.1000) |
| Antibody | Anti-human B-actin (rabbit monoclonal) | Cell Signaling | Cat# 8457,R RID:AB_10950489 | WB (1.2000) |
| Antibody | Anti-mouse CD44-APC (rat monoclonal) | BD Pharmingen | Cat# 559250, RRID:AB_398661 | FACS (1 µg/106 cells) |
| Antibody | Anti-human EpCAM-FITC (mouse monoclonal) | GeneTex | Cat# GTX30708, RRID:AB_1240769 | FACS (1 µg/106 cells) |
| Recombinant DNA reagent | ESRP1 cDNA ORF Clone (human) | Sino Biological | Cat# HG13708-UT | |
| Recombinant DNA reagent | pcDNA empty vector (plasmid) | Gift from Ron Smits | ||
| Recombinant DNA reagent | pcDNA-human-CD44s (plasmid) | Gift from Véronique Orian-Rousseau | ||
| Recombinant DNA reagent | pUC57-human-CD44v6 (plasmid) | Gift from Véronique Orian-Rousseau | ||
| Recombinant DNA reagent | pcDNA-human-NUMB1 (plasmid) | Gift from Salvatore Pece | ||
| Recombinant DNA reagent | pcDNA-human-NUMB2 (plasmid) | Gift from Salvatore Pece | ||
| Recombinant DNA reagent | pcDNA-human-NUMB3 (plasmid) | Gift from Salvatore Pece | ||
| Recombinant DNA reagent | pcDNA-human-NUMB4 (plasmid) | Gift from Salvatore Pece | ||
| Recombinant DNA reagent | shZEB1 | Sacchetti et al., 2021 | Cat# 1864 | |
| Recombinant DNA reagent | pSLIK-Hygro | Addgene | Cat# 25737 | |
| Software, algorithm | R | Seurat, GSVA, MAGIC (Stuart et al., 2019; van Dijk et al., 2018; La Manno et al., 2018) | RRID:SCR_007322, RRID:SCR_021058 | Version 4.0.4 |
| Software, algorithm | Python | Velocyto, scVelo (La Manno et al., 2018; Bergen et al., 2020) | RRID:SCR_018167, RRID:SCR_018168 | Version 3.8.3 |
| Software, algorithm | STAR | Dobin et al., 2013 | RRID:SCR_004463 | |
| Software, algorithm | MISO | Katz et al., 2010. | RRID:SCR_003124 |