Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-VP1 (Rat Clone 8A7H5) | Swimm et al., 2010 | Clone 8A7H5 | 250 µg/week |
| Antibody | ChromPure Rat IgG | Jackson ImmunoResearch | Cat#012-000-003 RRID: AB_2337136 |
250 µg/week |
| Antibody | Anti-CD8β (H35-17.2) | Golstein et al., 1982 | N/A | 250 µg/week |
| Antibody | Anti-CD4 (GK1.5) | Dialynas et al., 1983 | N/A | 250 µg/week |
| Antibody | Anti-VP1 (Rabbit polyclonal) | Provided by Robert Garcea | N/A | IF (1:1,000) FC (1:10,000) |
| Antibody | Anti-Vimentin (Rat Clone 280618) | R&D Systems | Cat#MAB2105 RRID: AB_2241653 |
IF (1:500) |
| Antibody | Anti-GFAP (Goat polyclonal) | Abcam | Cat#ab53554 RRID: AB_880202 |
IF (1:1,000) |
| Antibody | Anti-THP (Rat monoclonal) | R&D Systems | Cat#MAB5175 RRID: AB_2890000 |
IF (1:1,000) |
| Antibody | Anti-CD13 (Goat polyclonal) | R&D Systems | Cat#AF2335 RRID: AB_2227288 |
IF (1:500) |
| Antibody | Anti-Goat IgG AF488 (Bovine polyclonal) | Jackson ImmunoResearch | Cat#805-545-180 RRID: AB_2340883 |
IF (1:500) |
| Antibody | Anti-Rat IgG AF555 (Donkey polyclonal) | Abcam | Cat#ab150154 RRID: AB_2813834 |
IF (1:500) |
| Antibody | Anti-Rabbit IgG AF647 (Donkey polyclonal) | Jackson ImmunoResearch | Cat#711-605-152 RRID: AB_2492288 |
IF (1:500) |
| Antibody | Anti-CD8α-AF700 (Clone 53–6.7) | Biolegend | Cat#100730 RRID:AB_493703 |
FC (1:200) |
| Antibody | Anti-CD45-PerCP/Cy5.5 (Clone 30-F11) | Biolegend | Cat#103132 RRID:AB_893340 |
FC (1:200) |
| Antibody | Anti-CD45-FITC (Clone 30-F11) | BD | Cat#553080 RRID:AB_394610 |
FC (3 µg/mouse) |
| Antibody | Anti-CD4-PE (Clone RM4-5) | Biolegend | Cat#100512 RRID:AB_312715 |
FC (1:200) |
| Antibody | Anti-CD4-BV421 (clone GK1.5) |
BD | Cat#562891 RRID: AB_2737870 |
FC (1:200) |
| Antibody | Anti-Rat IgG-APC (Goat polyclonal) | BD | Cat#551019 RRID:AB_398484 |
FC (1:200) |
| Antibody | Anti-Mouse IgG-HRP (Goat polyclonal) | Bethyl Laboratories INC | Cat#A90-116P RRID:AB_67183 |
ELISA (1:7,000) |
| Other | Fixable Viability Dye eFluor 780 | ThermoFisher | Ref#65-0865-14 | FC (1:1,000) |
| Other | Flow Cytometry Absolute Count Standard | Bangs Laboratories | Cat#580 | |
| Other | MuPyV (Strain A2) | N/A | N/A | |
| Other | Sheep Red Blood Cells | Innovative Research | Cat#ISHRBC10P15ML | |
| Peptide, recombinant protein | VP1 pentamers | Provided by Robert Garcea | N/A | |
| Peptide, recombinant protein | Benzonase Nuclease | Sigma | Cat#E1014 | |
| Peptide, recombinant protein | Neuraminidase from Vibrio cholerae | Sigma | Cat#N6514 | IF (1:100) FC (1:200) |
| Peptide, recombinant protein | Collagenase (Type I) | Worthington | Cat#LS004197 | |
| Chemical compound, drug | TRIzol Reagant | ThermoFisher | Ref#15596018 | |
| Chemical compound, drug | RevertAid H Minus Reverse Transcriptase | ThermoFisher | Cat#EP0451 | |
| Chemical compound, drug | Lipofectamine 2000 Transfection Reagent | ThermoFisher | Cat#11668030 | |
| Chemical compound, drug | Lipofectamine 3000 Transfection Reagent | ThermoFisher | Cat# L3000008 | |
| Commercial assay, kit | PFHM-II Protein-Free Hybridoma Medium | ThermoFisher | Ref#12040–077 | |
| Commercial assay, kit | TOPO TA Cloning Kit | ThermoFisher | Ref#45–0641 | |
| Commercial assay, kit | TBP PrimeTime XL qPCR Assay | IDT | Mm.PT.39a.22214839 | |
| Commercial assay, kit | 1-Step Ultra TMB-ELISA | ThermoFisher | Ref#34028 | |
| Commercial assay, kit | 96 Well EIA/RIA Polystyrene High Bind Microplate | Fisher Scientific | Cat#3590 | |
| Commercial assay, kit | PerfectCTa FastMix II ROX | Quantabio | P/N 84210 | |
| Commercial assay, kit | PureLink Viral RNA/DNA mini Kit | ThermoFisher | Ref#12280–050 | |
| Commercial assay, kit | Wizard Genomic DNA Purification Kit | Promega | Ref#A1120 | |
| Commercial assay, kit | QuikChange II Site-Directed Mutagenesis Kit | Agilent | Cat#200523 | |
| Commercial assay, kit | CELLine Disposable Bioreactor | Fisher Scientific | Cat#353137 | |
| Cell Line (M. musculus) | BALB/3T3 Clone A31 | ATCC | CCL-163; RRID:CVCL_0184 | |
| Cell Line (M. musculus) | NMuMG | ATCC | CRL-1636; RRID:CVCL_0075 | |
| Strain, strain background (M. musculus) | C57BL/6 Mice | National Cancer Institute | Cat#OIC55 | |
| Genetic reagent (M. musculus) | μMT Mice | Jackson Laboratory | Cat#002288; RRID:IMSR_JAX:002288 | |
| Software, algorithm | Prism | Graphpad | v 9.3.1; RRID:SCR_002798 | |
| Software, algorithm | FlowJo | BD | v 10.6.1; RRID:SCR_008520 | |
| Software, algorithm | ImageJ | NIH | v 1.8.0; RRID:SCR_003070 | |
| Software, algorithm | Leica LAS X | Leica | v 3.7.2; RRID:SCR_013673 |