Table 1. Cryo-ET data collection and processing statistics.
IFT-A complex (EMD-26791) | |
---|---|
Data collection | |
Magnification | 30,000 |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | 100–140 |
Defocus range (μm) | –3 to –6 |
Pixel size (Å) | 14.13 (bin6), 7.08 (bin3) |
Tilt-range/step (°) | ±64° / 2 |
Processing | |
Symmetry imposed | C1 |
Final particle images (no.) | 9350 |
Map resolution (Å) | 23 |
FSC threshold | 0.5 |