Table 3.

Overall alignment of SARS-CoV-2, SARSr-CoV and MERS-CoV sequence conservation patterns of nsp12-RdRp domain. Analysis of nsp12 sequences from SARS-CoV-2, SARSr-CoV (SARS-CoV-2 and SARS-CoV) and SARSr-/MERS-CoV group at each amino acid position from the RdRp-domain according to its conservation. The druggable sites were predicted according to the results of two independent bioinformatic tools: DGSS and SF. Each site is coloured according to the conservation score scale (0-11) showed in this figure. Highly variable residues are coloured in red (scores 0–3), variable residues in pink (scores 4–6), conserved residues in light blue (scores 7–9) and highly conserved residues in dark blue (scores 10–11). Highly conserved, conserved, and variable residues represented 79.94%, 9.12% and 10.94%, respectively, considering all sequences in study. Distinct conservation patterns are mostly present in NiRAN domain, in which it was reported a decrease of the cs in 49.8% residues. Nevertheless, 68 were top-conserved in all grouped sequences, namely: S1, F7, L8, R19, V11, G13, R18, L19, G23, T28, C53, C54, R55, Q57, E58, E61, F70–N79, H82, I86, I171, L178, R181-Q184, L186, L187, V190, C193, A199, V202, G203, L205-Y217, F219, G220, D221, F222, V234, Y237, Y238, S239, L240, L241, M242, I244, L245, T246.

Coloured web version. If accepted, a black-and-white version of the figure will be provided for print.