Table 6.
Summary table of promising CoV-RdRp nsp12 protein residues identified in this study and respective experimental findings described in literature regarding their potential role on protein function/structure. Conservation results concern the analysis of all SARSr-/MERS-CoV sequences. Conservation score scale: 0–11. Druggability score scale: 0–2.
Coloured web version. If accepted, a black-and-white version of the figure will be provided for print.
| nsp12 Residue | nsp12 domain | Conservation/Druggability scores | C-CDP | Findings | Authors |
|---|---|---|---|---|---|
| K545 | Fingers | 11/2 | C-CDP10 | Interaction with molnupiravir. | Seahan et al. (Sheahan et al., 2020) |
| A547 | 11/2 | ||||
| I548 | 11/2 | ||||
| S549 | 11/2 | ||||
| R555 | 11/2 | ||||
| K545 | Fingers | 11/2 | Residues from the NTP entry channel (motif F) | Gao et al. (Gao et al., 2020) | |
| R553 | 11/2 | C-CDP10 | |||
| R555 | 11/2 | ||||
| D618 | Palm | C-CDP17 | Divalent cation-binding residue | Gao et al. (Gao et al., 2020) | |
| S759 | Palm | 11/0 | – | SDD region (motif C) | Gao et al. (Gao et al., 2020) |
| D760 | 11/1 | – | |||
| D761 | 11/2 | C-CDP17 | |||
| K500 | Fingers | 11/0 | C-CDP9 | Essential for nsp12 protein conformational rearrangements (motif G) | Schumer-Furmer et al. (Schueler-Furman and Baker, 2003) |
| S501 | 11/2 | ||||
| G502 | 11/2 | ||||
| K545 | Fingers | 11/2 | C-CDP10 | Favipiravir binding site in the RdRp active site | Naydenova et al. (Naydenova et al., 2020) |
| K551 | 10/2 | ||||
| R553 | 11/2 | ||||
| D618 | Palm | 11/2 | C-CDP17 | ||
| K621 | Fingers | 11/2 | C-CDP8 | ||
| D761 | Palm | 11/2 | C-CDP17 | ||
| K798 | 11/2 | C-CDP17 |