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. 2022 Jul 26;146(21):1591–1609. doi: 10.1161/CIRCULATIONAHA.121.057623

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© 2022 The Authors.

Circulation is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 2. — VSMC-specific NR1D1 deficiency represses AngII-induced AAA formation. A, Schematic protocol: ApoE^−/−/Nr1d1^flox/flox and ApoE^−/−/Nr1d1^ΔSMC mice were subcutaneously injected with saline or AngII by a mini osmotic pump for 28 days (n=30 per group). B, Representative images of the macroscopic features of AAA formation in indicated groups. C, Representative images of macroscopic features and HE staining of crossed-sections of aneurysm ruptures (arrow indicated). D, Survival curves in indicated groups (n=30 per group). Survival data were analyzed by the Kaplan-Meier method and compared using log-rank tests. *P<0.05. E, The incidence of AngII-induced AAA in indicated groups (n=30 per group). Data were analyzed by a Fisher exact test. **P<0.01; ***P<0.001. F, The incidence of AngII-induced aneurysm rupture in indicated groups (n=30 per group). Data were analyzed by a Fisher exact test. *P<0.05. G and H, Quantification of the maximal diameter of suprarenal abdominal aortas measured by a Digital Vernier Caliper and total aortic weight/BW in indicated groups (n=22–30 per group). Data were analyzed by 2-way ANOVA followed by the Bonferroni post hoc test. ***P<0.001. I, Representative images of abdominal aortas visualized by MUI using the B mode in indicated groups. J, Quantification of the maximal diameter of suprarenal abdominal aortas measured by MUI using the B mode in indicated groups (n=22–30 per group). Data were analyzed by 2-way ANOVA followed by the Bonferroni post hoc test. ***P<0.001. K, Representative images of abdominal aortas visualized by MRI in indicated groups. Top, Abdominal aortas visualized using 3-dimensional time of flight fast low angle shot sequence (TOF-3D-Flash). Bottom, Abdominal aortas visualized by T2-weighted, PD-weighted imaging with multiple-echo multishot sequence (MEMS-PD-T2). Data are expressed as mean ± SEM. AAA indicates abdominal aortic aneurysm; AngII, angiotensin II; BW, body weight; EVG staining, elastin van Gieson staining; HE staining, hematoxylin and eosin staining; MUI, micro-ultrasound imaging; MRI, magnetic resonance imaging; NR1D1, nuclear receptor subfamily 1 group D member 1; and SMC, smooth muscle cell.