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. 2022 Nov 14;8(11):e11622. doi: 10.1016/j.heliyon.2022.e11622

Figure 2.

Figure 2

Effects of different concentrations of acteoside on the inflammatory mediator expression of HMEC induced by CAC. HMEC were pre-treated with different concentrations (20, 10 and 5 μg/mL) of acteoside for 2 h and then incubated with 30 μL of cobra venom factor (CVF)-activated complement (CAC) products for 48 h. Interleukin-6 (A, IL-6) and tumor necrosis factor-α (B, TNF-α) in the medium were determined by enzyme-linked immunosorbent assay (ELISA). Values represented the means ± SEM (n = 3). #P < 0.05 and ##P < 0.01 were compared with the control group; ∗P < 0.05 and ∗∗P < 0.01 were compared with the CVF group.