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. 2022 Nov 18;12:19891. doi: 10.1038/s41598-022-24178-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Slurry shows rapid onset reduction in nerve function (A) Paw withdrawal frequency in response to stimulation with 15 g VFH force at different time points post slurry injection. (B) Effects of slurry injection on paw withdrawal latency in response to heat stimulation in treated hind limbs. (C) Toe spread and (D) walking scores of hind limbs treated with slurry. Data are presented as median with interquartile range. n = 9; * P < 0.05, ** P < 0.001 compared to baseline values by Friedman test followed by Dunn’s multiple comparisons test. (E) CARS microscopy images show myelin and nerve structure in sciatic nerve samples after injection of slurry and room temperature control solution. (F) Graph shows CCP index after injection of slurry and room temperature control solution. Data are presented as mean ± SD. n = 4 per group; * P < 0.0001 compared to baseline values by Ordinary one-way ANOVA followed by Tukey's multiple comparisons test; RT: room temperature.