Table 1.
Effect of temperature on the relevant mechanical parameters listed on the first row.
| Temperature (°C) | T0,4.5 (kPa) | s0 (nm) | F0 (pN) | nH | pCa50 | |
|---|---|---|---|---|---|---|
| soleus fibre | ||||||
| control | ||||||
| 12.3 ± 0.1 (14) | 127 ± 5 | 3.16 ± 0.12 | 1.71 ± 0.06 | 1.97 ± 0.07 | 6.38 ± 0.05 | |
| 17.3 ± 0.1 (4) | 148 ± 12 | 3.78 ± 0.25 | 2.04 ± 0.14 | 2.42 ± 0.09 | 6.54 ± 0.06 | |
| 25.2 ± 0.1 (4) | 165 ± 12 | 4.53 ± 0.20 | 2.45 ± 0.11 | 3.15 ± 0.13 | 6.55 ± 0.03 | |
| 35.5 ± 0.1 (5) | 183 ± 3 | 5.34 ± 0.32 | 2.59 ± 0.19 | 3.09 ± 0.20 | 6.67 ± 0.09 | |
| 1 µM OM | ||||||
| 12.2 ± 0.1 (5) | 69 ± 3 | 1.53 ± 0.05 | 0.83 ± 0.06 | 0.79 ± 0.09 | 7.17 ± 0.09 | |
| 25.2 ± 0.1 (5) | 117 ± 14 | 2.75± 0.34 | 1.48 ± 0.18 | 0.96 ± 0.13 | 7.21 ± 0.10 | |
| 35.6 ± 0.1 (5) | 134 ± 14 | 3.11 ± 0.30 | 1.68 ± 0.16 | 1.17 ± 0.08 | 7.16 ± 0.08 | |
| psoas fibre | ||||||
| 6.0 ± 0.1 (4) | 135 ± 6 | 1.71 ± 0.11 | 2.90 ± 0.18 | 3.51 ± 0.22 | 5.89 ± 0.05 | |
| 12.1 ± 0.1 (12) | 219 ± 11# | 2.86 ± 0.16# | 4.87 ± 0.27 | 3.92 ± 0.21 | 6.00 ± 0.13 | |
| 25.0 ± 0.1 (6) | 317 ± 14# | 4.13 ± 0. 19# | 7.02 ± 0.32 | 4.81 ± 0.22 | 6.46 ± 0.06 |
Data for soleus fibres in control and in the presence of 1 µM OM. For comparison also data for psoas fibres are reported (from ref. 33 and new data). Data are mean ± SEM. In brackets the number of fibres used for each temperature. # mean calculated by pooling data from new experiments with data from ref. 33. In each of the three cases the statistical significance of the temperature effect is tested with one-way ANOVA as detailed in Supplementary Table 2. The soleus values in control of F0 and nH at 35.6 °C are not significantly different from the corresponding psoas values at 6.0 °C (P = 0.24 and 0.16 respectively).