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. 2022 Nov 18;12:19896. doi: 10.1038/s41598-022-23961-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The uptake of tobacco vesicles by tobacco cells. (A) Vesicles isolated from callus with no trehalose added, (B) vesicles isolated from callus, pellet resuspended in PBS with trehalose, (C) samples isolated from BY-2, no trehalose added, (D) sample isolated from BY-2, trehalose was added for pellet resuspension. Samples (E) (callus) and (F) (BY-2) were isolated using PEG precipitation.