Table 1.
The characterization of pEVs and PDNVs isolated by ultracentrifugation from BY-2 and callus cultures of tobacco.
| Ultracentrifugation | DLS | NTA | NTA | BCA | |
|---|---|---|---|---|---|
| Sample origine | Trehalose | Z-AverageDiameter (nm) | Size (nm) | Concentracion (particles/ml) | Protein concentration (μg/ml) |
| Callus | X | 631.4 ± 158.0 (□) | 209.3 ± 10.85 | 1.71 × 109 ± 3.46 × 108 | 96.19–506.18 |
| P | 380.4 ± 136.70 (*) | 214.7 ± 9.26 | 3.61 × 109 ± 1.50 × 109 | 98.14–482.86 | |
| B | 967.6 ± 63.14 (*, ○) | 253.2 ± 25.31 | 1.86 × 109 ± 1.61 × 109 | 179.04–486.50 | |
| BY-2 suspension culture | X | 565.1 ± 220.7 | 175.5 ± 14.45 | 4.69 × 109 ± 1.06 × 109 | 713.60–1147.06 |
| P | 354.2 ± 66.85 (∆) | 301.9 ± 115.90 | 7.98 × 109 ± 4.52 × 109 | 732.28–1046.90 | |
| B | 681.0 ± 143.30 (⌂) | 352.8 ± 92.57 | 2.18 × 109 ± 1.80 × 109 | 552.75–983.61 | |
The comparison of the vesicles sizes using Dynamic Light Scattering (DLS) and Nanoparticle Tracking analysis (NTA) and protein concentration. X—no trehalose added, P—100,000 × g ultracentrifugation pellet resuspended in 25 mM trehalose in PBS, B—25 mM trehalose added at the beginning of the isolation and also for the resuspension of 100,000 × g ultracentrifugation pellet. Marks (□,*, ○, ∆, ⌂) written behind the Z-Average values indicate samples which Z-Average size differences are significant.