Table 2.
The characterization of pEVs and PDNVs isolated by polyethylene glycol precipitation from BY-2 and callus cultures of tobacco.
| PEG precipitation | DLS | NTA | NTA | BCA | |
|---|---|---|---|---|---|
| Sample origin | Trehalose | Z-average diameter (nm) | Size (nm) | Concentration (particles/ml) | Protein concentration (μg/ml) |
| Callus | X | 116.70 ± 8.67 (□, ○) | 209.9 ± 0.38 | 0.55 × 109 ± 1.75 × 108 | 1843.69 – 2073.56 |
| BY-2 suspension culture | X | 138.70 ± 32.80 (∆, ⌂) | 205.6 ± 10.84 | 2.63 × 109 ± 1.25 × 109 | 106.24 – 318.78 |
The comparison of the vesicle size using Dynamic Light Scattering (DLS) and Nanoparticle Tracking analysis (NTA) and protein concentration. X—no trehalose added.