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Effects of STE on NF-κB pathway. The expression level of IκBα in the cytoplasm and p65 in the nuclei was determined via western blot analysis (A–C). Immunofluorescence and DAPI staining of nuclei to assess nuclear translocation of p65 (scale bar: 10 μm) (D). Data were expressed as mean ± SD. Significant differences between different groups are shown as ##P < 0.01, vs control group; ∗∗P < 0.01, vs IL-1β alone stimulation group, n = 3.
