Figure 10.

E₉₁NP₃vaccination reduces Mtb burden in the lungs. The mice were immunized (i.n.) with E₉₁NP₃. Later, two booster doses were given at an interval of 14 days. Two weeks after final immunization, the mice were aerosol-challenged with Mtb-H37Rv. Finally, after 25 days post-infection, the animals were sacrificed to enumerate Mtb burden in the lungs. A, schematic diagram showing the time points of immunization, infection, and experimentation. B, bar graph shows the colony-forming unit count/gram lung tissue for each group of mice. The data (mean ± SE) shown are representative of two experiments with three mice per group. ∗∗p < 0.01. Mtb, Mycobacterium tuberculosis; NP, nanoparticle.