Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 15;298(12):102596. doi: 10.1016/j.jbc.2022.102596

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Conjugation of P₃on the surface of E₉₁NP. E₉₁NPs were used to conjugate P₃ on the surface using EDC–NHS chemistry. The detailed protocol for conjugation has been described in the Experimental procedures section. The conjugation was confirmed by several techniques. A and B, DLS plots show the changes in (A) ζ-potential (data compared with ζ-potential of blank NPs from Fig. 1A); (B) DLS size before and after P₃ conjugation (data compared with a hydrodynamic diameter of blank NPs from Fig. 1B); (C) FTIR spectra showing the presence of C=O stretch of saturated ester (1742 cm⁻¹) and 2° amide (1680 cm⁻¹), imparted by the conjugation of P₃ molecules on the surface of NPs. Biotinylated P₃ (bio-P₃) was used for isothermal titration calorimetry (ITC). D, heat flow profile of ITC exhibiting time upon injection of HRP–streptavidin to NPs surface-functionalized with either (i) P₃ or bio-P₃. DLS, dynamic light scattering; EDC, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride; HRP, horseradish peroxidase; NHS, N-hydroxylsuccinimide; NP, nanoparticle; ζ-potential, zeta potential.

Conjugation of P₃on the surface of E₉₁NP. E₉₁NPs were used to conjugate P₃ on the surface using EDC–NHS chemistry. The detailed protocol for conjugation has been described in the Experimental procedures section. The conjugation was confirmed by several techniques. A and B, DLS plots show the changes in (A) ζ-potential (data compared with ζ-potential of blank NPs from Fig. 1A); (B) DLS size before and after P₃ conjugation (data compared with a hydrodynamic diameter of blank NPs from Fig. 1B); (C) FTIR spectra showing the presence of C=O stretch of saturated ester (1742 cm⁻¹) and 2° amide (1680 cm⁻¹), imparted by the conjugation of P₃ molecules on the surface of NPs. Biotinylated P₃ (bio-P₃) was used for isothermal titration calorimetry (ITC). D, heat flow profile of ITC exhibiting time upon injection of HRP–streptavidin to NPs surface-functionalized with either (i) P₃ or bio-P₃. DLS, dynamic light scattering; EDC, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride; HRP, horseradish peroxidase; NHS, N-hydroxylsuccinimide; NP, nanoparticle; ζ-potential, zeta potential.