Figure 6.

Mechanism of E₉₁NP₃uptake by DCs. The DCs were preincubated with inhibitors of dynasore hydrate (10–80 μM), genistein (25–200 μM), or cytochalasin D (0.625–5 μM) for 3 h. Later, DCs were incubated with E₉₁NP₃-FITC or medium alone (control) for 24 h. The DCs were harvested and stained for flow cytometry. A, the histogram and (B) bar graph show the uptake of E₉₁NP₃-FITC by DCs. Histograms are representative of two independent experiments, and the bar graph denotes the mean of two different experiments. The greyline in the bar diagram signifies the drift in E₉₁NP₃-FITC uptake with the increasing doses of the concerned inhibitors. DC, dendritic cell; NP, nanoparticle.