Figure 7.

Intracellular trafficking of E₉₁NP₃in DCs. The DCs were cultured with E₉₁NP₃-FITC. At 1, 2, 4, and 6 h post-culture, the cells were stained with DAPI (blue) and fluorochrome-labeled anti-LAMP1 Abs. A, confocal images depict E₉₁NP₃-FITC, LAMP1, and colocalization (arrow) of E₉₁NP₃-FITC and lysosomes (yellow) at 1, 2, 4, and 6 h. The nucleus is shown as blue. The formula used for the calculation of the percentage of colocalization is also shown. B, the line graph depicts the gradual trafficking of the E₉₁NP₃-FITC to the lysosome in a time-dependent manner. Data shown at each time point are from 50 ± 5 individual cells and two independent experiments. Ab, antibody; DAPI, 4′,6-diamidino-2-phenylindole; DC, dendritic cell; LAMP1, lysosomal-associated membrane protein 1; NP, nanoparticle.