Figure 3.

Vimentin elongation measured with AFM. (A) Vimentin filaments imaged with AFM after 10, 90, and 180 s after assembly start. Scale bar is 1 μm. (B) Geometric mean of filament length versus assembly time. The black line indicates a linear fit to the data, with a slope of 1.02 ULFs/min. (C) Distribution of filament lengths at different time points after starting assembly. Points indicate measurements of probability density from AFM images in logarithmically spaced bins (0.5–1 ULF, 1–2 ULFs, 2–4 ULFs, etc.); lines are a log-normal fit to the data. (D) Geometric standard deviation of filament length distribution versus geometric mean filament length. The black line indicates a linear fit to the data, with $\sigma =0.053l_{\mathrm{f}\mathrm{i}\mathrm{l}}+0.115$, when the filament length $l_{\mathrm{f}\mathrm{i}\mathrm{l}}$ is given in units of #ULFs. To see this figure in color, go online.