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(A–C) Assembly of vimentin filaments (0.2 mg/mL in 50 mM NaCl) at 37°C for 10 (A), 30 (B), and 180 s (C). Transmission electron microscopy of glutaraldehyde-fixed and uranyl acetate negatively stained specimens. Scale bar: 200 nm. (D) Filament diameter and distribution at various time points. Red points represent individual measurements, blue points show the average, and black bars denote standard deviation. To see this figure in color, go online.
