Figure 5.

(A) Monte Carlo simulation of the ratio of the scattered light intensities at 594 and 405 nm for different longitudinal assembly rates and time points after starting the assembly (gray curves). The filament length and its distribution versus time is assumed to follow the relationship shown in Figs. 2B and 3D. The orange lines represent the fit of an exponential function (Eq. 6) to the data. The time constant τ of the exponential fit scales inversely with the longitudinal assembly rate $r_{la}$, according to $r_{la}$= 2.32 ULFs/τ, if τ is given in units of min (inset). (B) Scattered light intensities at 594 (orange) and 405 nm (blue) over time for assembly of 0.2 mg/mL vimentin in low-, medium-, and high-salt buffer. The scattered light intensities at 405 and 594 nm develop synchronously but begin to diverge beyond 10 s due to the wavelength-dependent scattering properties of elongated filaments. Representative measurements for all other conditions are shown in Fig. S3. (C) Scattered light intensity ratios (594/405 nm) for 0.2 mg/mL vimentin (blue) measured in low-, medium-, and high-salt buffer, with exponential fits (Eq. 6; gray). Representative curves for all other conditions are shown in Fig. S3. To see this figure in color, go online.