Figure 7.

(A and B) Light scattering signal at 405 (top row) and 594 nm (bottom row) during assembly of 0.2 mg/mL vimentin in 100 mM (green) and 160 (orange) salt, normalized by the signal measured for 50 mM salt. (C and D) Signal ratio (green: 100 mM/50 mM; orange: 160 mM/50 mM) as shown in (A) and (B) averaged between 20 and 40 ms after assembly start, reporting differences in the lateral assembly kinetics relative to the assembly at low-salt conditions. (E and F) Ratio averaged over a 40 s wide interval around the intensity peak, combining contributions from lateral and longitudinal assembly. (G and H) Ratio averaged between 500 and 600 s after assembly start, reporting differences in the mass per cross section of the filaments relative to filaments assembled in 50 mM salt buffer. Square symbols show mean ± SE, circles show individual measurements from independent experiments. To see this figure in color, go online.