Table 3.
Isothermal titration calorimetry of insulins against IR485 with an αCT peptidea
| Insulin analog | αCT peptide | KD (nM) | ΔH (kJ mol−1) | TΔS (kJ mol−1) |
|---|---|---|---|---|
| HI | IR-A αCT704–719 | 30.5 ± 5.4 | -63.6 ± 9.6 | −20.5 ± 9.2 |
| IR-B αCT704–731 | 37.3 ± 5.3 | −58.3 ± 2.1 | −15.1 ± 2.1 | |
| SCI-b | IR-A αCT704–719 | 20.1 ± 8.4 | −90.0 ± 0.4 | −45.6 ± 1.3 |
| IR-B αCT704–731 | 160.6 ± 6.5 | −94.6 ± 1.7 | −56.1 ± 1.7 |
Data are from three technical replicates; errors are SEM. For further details, see materials and methods.