Table 3.

Structure–Activity Relationship (SAR) on the 4-Sulfamoylbenzamide Moiety with the Implementation of Various Potential Hinge-Binders for the Development of Type II Inhibitors^a

^aBinding of the substituted sulfamoylbenzamide derivatives (compounds 33–40 and 48) to LIMK1/2 as determined by DSF. ΔTm shifts are reported as means of three independent experiments. IC₅₀ values for LIMK1 and LIMK2 were determined in a cellular assay system using NanoBRET. IC₅₀ values are reported as means ± SD of three independent experiments.