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. Author manuscript; available in PMC: 2022 Nov 19.
Published in final edited form as: Nat Mater. 2013 Oct 20;12(12):1154–1162. doi: 10.1038/nmat3777

Fig. 3. Microtopography reduced HDAC activity, downregulated HDAC2, and upregulated WDR5.

Fig. 3.

For these experiments, mouse fibroblasts were analyzed after being cultured on flat or microgrooved surfaces for 3 days. a, Nuclear HDAC activity assay (n=3). * p<0.05 (two-tailed, unpaired t-test) compared with the control flat surface. b, Western blotting (WB) analysis of HDACs. c, Confocal microscopy of HDAC2 staining. d, WB analysis of WDR5. e, Confocal microscopy of WDR5. f, WB analysis performed on fibroblasts cultured on microgrooves after siRNA knockdown of WDR5. (Scale bars in c and e, 10 μm)