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. 2022 Nov 19;20:159. doi: 10.1186/s12958-022-01036-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Localization of TARID in placenta and trophoblasts and the transfection efficiency. a Representative photomicrographs of TARID fluorescence in situ hybridization (FISH) (400×). b The subcellular localization of TARID in HTR8/SVneo cells was detected by FISH assays (2000×). Red, TARID; blue, nucleus. Scale bar, 20 mm. c The subcellular localization of TARID in the HTR8/SVneo cells detected by cell fractionation assays. U6, nucleus marker; β-actin, cytoplasm marker. All experiments were conducted in triplicate; the values are shown as the mean ± SEM; vs. vector or ASO，**P < 0.01, *P < 0.05