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. 2022 Nov 19;20:159. doi: 10.1186/s12958-022-01036-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — TARID promoted tube formation in trophoblasts but was not involved in proliferation and apoptosis. a Tube formation was detected in HTR8/SVneo cells after TARID overexpression and knockdown (400×). Left panel: photos of tube formation; right panel: statistics of cells. b, c Cell Counting Kit-8 was used to evaluate the cell proliferation of HTR8/SVneo (b) JAR (c) cells after TARID overexpression and knockdown. d, e Apoptosis analysis using flow cytometry on HTR8/SVneo (d) and JAR (e) cells after TARID overexpression and knockdown. Upper panels: histograms of apoptotic cells; lower panels: statistics of apoptotic cells. All experiments were conducted in triplicate; the values are shown as the mean ± SEM; vs. vector or ASO，**P < 0.01, *P < 0.05