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. 2022 Nov 19;23:316. doi: 10.1186/s12931-022-02240-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The functional activity determination of the trivalent IL-5-HSA Nb. A The binding activity of the trivalent IL-5-HSA Nb towards human IL-5 and cyno IL-5. B The binding activity of the trivalent IL-5-HSA Nb towards HSA and CSA at pH 5.5 and pH 7.4. The binding activity was detected by ELISA. C The blocking effect of trivalent IL-5-HSA Nb or Nucala analogue on the IL-5/IL-5R interaction was determined by flow cytometry. D The inhibitory effect of trivalent IL-5-HSA Nb or Nucala analogue on TF-1-cell proliferation was determined by the CCK8 assay