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Local humoral response against 9B peptide-1 in the lungs of immunized mice. C57BL/6J mice were immunized twice i.n. with recombinant 9B-expressing flagella (▴) or with native flagella (▵). Their lungs were removed, homogenized in PBS, and tested by ELISA for the IgA response against 9B peptide-1 conjugated to BSA. O.D., optical density.
