TABLE 1.

Protection of mice from infection by S. mansoni after i.n. vaccination with recombinant 9B peptide-1-expressing flagella, in comparison to f.p. immunization^a

Expt no.	Route	Antigen	No. of worms (mean ± SE)	% Protectionb
1	i.n.	Fla-9B	25.88 ± 2.33	30*
		Native Fla	35.85 ± 2.31	2
		None	36.57 ± 4.70	0
2	i.n.	Fla-9B	6.25 ± 2.06	53*
		Native Fla	18.37 ± 2.87	0
		None	13.50 ± 1.33	0
3	i.n.	Fla-9B	27.40 ± 3.22	42*
		Native Fla	36.33 ± 3.00	4
		None	38.00 ± 4.00	0
4	f.p.	Fla-9B	24.40 ± 1.59	32*
		Native Fla	35.00 ± 2.87	0
		None	34.00 ± 1.00	0
5–8	f.p.	BSA-9B	17.75 ± 2.75	39*
		BSA	32.00 ± 3.55	0
		None	29.33 ± 1.15	0

^aIn three replicate experiments, 8 to 10 C57BL/6J mice per group were immunized i.n. three times with either recombinant (Fla-9B) or native (Native Fla) flagella, and 4 weeks later they were infected with cercariae. At 7 to 8 weeks after infection, liver perfusion was performed and the number of adult worms was counted. 

^bCalculated from the number of worms in the immunized mice compared to that in the untreated mice. *, P < 0.05.