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. 2022 Nov 15;16:3929–3946. doi: 10.2147/DDDT.S377001

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© 2022 Rong et al.

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The Wnt signaling inhibitor XAV939 reversed the effects of ED-71. (A). ALP staining of MC3T3-E1 cells in OM, DEX, DEX+XAV939, DEX+ ED-71, DEX+ED-71+XAV939 groups at 7 days. Bar, 500μm. (B). AR staining images of these groups after 21 days of culture. Bar, 500μm. (C). The mRNA levels of COL1, ALP, RUNX2, OCN and β-catenin detected by RT-qPCR after 7 days of culture. GAPDH was used as an internal control. (D). Western blotting detection of β-catenin, RUNX2, OCN, Wnt5a, Active β-catenin, FZD4 and GAPDH after 14 days of culture. (E). The statistical analysis of Western blotting. Data were given as the mean ± S.D. from three independent experiments. *P < 0.05, **P < 0.01. ***P < 0.001.

Abbreviations: OM, osteoblast inducing conditional media; DEX, dexamethasone; DEX+XAV939, dexamethasone+XAV939; DEX+ED-71, dexamethasone+eldecalcitol; DEX+ED-71+XAV939, dexamethasone+eldecalcitol+XAV939.