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. 2022 Nov 16;10:e14373. doi: 10.7717/peerj.14373

Table 4. Summary of the DoxA purification.

Step Total protein (mg)a Target protein (mg)b Purity (%)c
Ni-NTA 6734.6 48.9* 0.725
Size-exclusion chromatography 50.9 14.3 28.12
Ion exchange chromatography 7.9 1.5 19.48

Notes.

a

Protein concentration determined by Bradford assay using BSA as a standard protein.

b

Determined from total protein concentration and purity.

c

Purity determined by densitometric assessment of SDS-PAGE.

*

Recombinant protein.