Table 4. Summary of the DoxA purification.
Step | Total protein (mg)a | Target protein (mg)b | Purity (%)c |
---|---|---|---|
Ni-NTA | 6734.6 | 48.9* | 0.725 |
Size-exclusion chromatography | 50.9 | 14.3 | 28.12 |
Ion exchange chromatography | 7.9 | 1.5 | 19.48 |
Notes.
Protein concentration determined by Bradford assay using BSA as a standard protein.
Determined from total protein concentration and purity.
Purity determined by densitometric assessment of SDS-PAGE.
Recombinant protein.