TABLE 4.
Strain | LDH retention (%) bya:
|
Trypan blue exclusion (%) fromb:
|
||
---|---|---|---|---|
Macrophages | HEp-2 cells | Macrophages | HEp-2 cells | |
W22703 (pYV+, biotype 2) | 84.6 ± 4.5 | 72.1 ± 4.4 | 78.7 ± 3.3 | 76.2 ± 4.8 |
W22703c (pYV−, biotype 2) | 98.5 ± 0.9 | 97.8 ± 1.4 | 98.7 ± 1.3 | 99.1 ± 0.6 |
937 (clinical, biotype 1A) | 99.9 ± 0.1 | 99.9 ± 0.1 | 99.9 ± 0.1 | 99.9 ± 0.1 |
AM5 (nonclinical, biotype 1A) | 99.9 ± 0.1 | 99.9 ± 0.1 | 99.9 ± 0.1 | 99.9 ± 0.1 |
The percentage of LDH retention by cells incubated with bacteria is expressed as the percentage of LDH retained by uninfected control cells. Results are expressed as mean ± standard deviation of at least three independent determinations. The assay and the equation for calculating the amount of LDH released are provided in Materials and Methods.
Data are the percentage of macrophages and HEp-2 cells incubated with bacteria that excluded trypan blue and are the mean ± standard deviation of at least three independent determinations.