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. 2022 Nov 8;7(21):e157034. doi: 10.1172/jci.insight.157034

Figure 2. The wP prime leads to stronger GC and memory B cell responses in dLNs.

Figure 2

(A) AID-Cre-EYFP mice primed either with aP or wP vaccines or controls injected with alum (ctr) received 2 doses of tamoxifen at days 7 and 10 after prime vaccination. Mice were analyzed at days 14 and 30. (B) B220+EYFP+ live cells from dLNs of mice primed with aP and wP vaccines were distinguished into GC (GL7+) and memory (GL7–) B cells, by flow cytometry. (C) Total EYFP+ GC and EYFP+ memory B cell counts in the 2 dLNs are shown in the graphs. (D) A representative flow cytometry profile of heavy chain isotype distribution among EYFP+GL7+ B cells is shown for the aP and wP conditions at day 30 after prime. (E) IgM/IgD, IgG1, IgG2, and IgA distribution in the EYFP+ GC and memory subsets from mice analyzed at day 30 after prime are shown in the plots. Each point represents an individual mouse analyzed at day 14 or day 30 after prime vaccination. At least 2 independent experiments were performed for each analysis. Means (±SEM) are shown. Kruskal-Wallis analysis with uncorrected Dunn’s test was performed to compare the different conditions at each time point or each Ab isotype. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.