Figure 8. Formation of large numbers of Bp-specific long-lived PCs in the BM requires 1 wP vaccination.

AID-Cre-EYFP mice were primed and boosted (day 30) with homologous and heterologous combinations of the aP and wP vaccines or injected with alum (ctr). Three doses of tamoxifen were administrated at days 7, 10, and 31. Mice were analyzed 50 days after boost injection. (A) Cell numbers and isotype distribution of EYFP⁺ PCs assessed by flow cytometry in BM are shown in the graphs. (B) Numbers of IgG1⁺ or IgG2⁺ ASCs against sonicated Bp were determined by ELISPOT performed on total BM cells. Each point in the graphs represents an individual mouse. At least 2 independent experiments were performed for each analysis. Means (±SEM) are shown. Kruskal-Wallis analysis with uncorrected Dunn’s test was performed to compare the different conditions. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.