Figure 9. Production of Bp-specific Abs with broader antigen and isotypic diversity in recall responses is favored by combining wP and aP vaccines.

AID-Cre-EYFP mice were primed and boosted (day 30) with homologous and heterologous combinations of the aP and wP vaccines or injected with alum (ctr). Blood was collected at 5, 25, 40, and 50 days after boost injection. IgG1 and IgG2b Ab titers against pooled proteins (PT, PRN, FHA, Fim2,3) (A) or IgG1, IgG2b, IgG2c, and IgG3 Ab titers against sonicated Bp (B) were detected by ELISA from serum of vaccinated and control mice. Ab titers are arbitrary values and each point in the graphs represents data from an individual mouse. At least 2 independent experiments were performed for each analysis. Means (±SEM) are shown. Kruskal-Wallis analysis with uncorrected Dunn’s test was performed to compare the different conditions at each time point and the different time points between the same condition. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.