Figure 8. Senotherapy restores multiple deficiencies in DM1 fruit flies in vivo.

(A) Locomotor activity obtained in REC2 Drosophila model of DM1 (n = 53) and controls (n = 46) at 25 days. (B) Survival curve of DM1 flies and controls. Median is 30 versus 71 days: P = 0.0001. (C) mRNA levels of Dacapo and Psc in thorax of DM1 and control fruit flies (n ≥ 4, each point represents a pool of 12 flies). (D) mRNA levels of indicated genes in DM1 and control fruit flies (n ≥ 4). (E–G) mRNA levels of indicated genes in DM1 and control fruit flies (n ≥ 4) in the absence or presence of 5 mM Quercetin. (H) Locomotor activity obtained in REC2 Drosophila DM1 model nontreated (n = 55) or in presence of 5 mM Quercetin (n = 46) and 50 nM Dasatinib (n = 9) at the indicated time points. (I) Locomotor activity of nontreated DM1 (n = 9) and control (n = 6) flies or in the presence of 5 mM Quercetin (control, n = 7; DM1, n = 9) and 50 nM Dasatinib (control, n = 9; DM1, n = 8), respectively. (J) Survival curve of non-treated control (n = 45) and DM1 (n = 53) flies or in presence of 1 mM (DM1, n = 45; control, n = 50) and 5 mM Quercetin (DM1, n = 70; control, n = 45). P <0.0001 in the 2 doses of Quercetin in DM1 flies. Median in WT was 71, 77, and 76 days in nontreated, Q1, and Q5, respectively, and median in DM1 was 30, 59, and 53 in nontreated, Q1, and Q5, respectively. (K) Survival curve of DM1 flies nontreated (n = 53) or in presence of 5 mM Quercetin (n = 70) and 50 nM Dasatinib (n = 36). Median is 30 versus 59 versus 47 days and P < 0.0001 with both senolytics. P values were calculated using the Student’s t test with P value corrected for FDR, except for survival curves, which were completed with log-rank test. *P < 0.05, **P < 0.01, ***P < 0.001.