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. 2022 Nov 8;7(21):e163161. doi: 10.1172/jci.insight.163161

Figure 4. Glutamine improves kidney function upon IRI by modulating Tgm2-HSP70 interaction.

Figure 4

WT mice were subjected to sham or IRI surgery and received glutamine or saline. Western blotting was performed to assess the expression of Tgm2 levels (A and B). MALDI-IMS of kidney sections was performed in order to analyze Tgm2 distribution (C). In addition to glutamine or saline exposure, mice obtained Tgm2 inhibitor ERW1041E or DMSO as vehicle control by an intravenous injection (D–F). Plasma creatinine levels (D, n = 5) and neutrophil recruitment into the kidney (E, n = 5) as well as Tgm2 activity in kidneys (F, n = 4) were determined 12 hours after IRI induction. WT and conditional Tgm2-KO mice (Tgm2fl/fl sGLT2cre+) were subjected to IRI surgery. Plasma (G, n = 3) and urine creatinine (H, n = 3) as well as neutrophil recruitment (I, n = 3) and the renal tubular injury score (J, n = 3) were assessed 24 hours after IRI. Mean ± SEM, 1-way ANOVA *P < 0.05; **P < 0.005; ***P < 0.001.