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. 2022 Nov 8;7(21):e160854. doi: 10.1172/jci.insight.160854

Figure 3. β Cell proliferation was increased during puberty in humans.

Figure 3

β Cell proliferation in postmortem pancreatic sections of male (M) and female (F) children from 8 to 15 years old as assessed by immunofluorescent staining for MKI67 and C-peptide (CPEP). (A–C) Representative sections showing CPEP (red), MKI67 (green), and nuclei (DAPI, blue) from Tanner stages I and II (8-year-old female and 10-year-old male) and Tanner stage V (15-year-old female) (A), and Tanner stages III and IV (13-year-old female and 14-year-old male) (B and C), as indicated. Arrows show positive nuclei for MKI67. Scale bars: 50 μm. (D) β Cell proliferation presented as a percentage of MKI67+CPEP+ cells over CPEP+ cells grouped according to Tanner stage in males (black square) (n = 5) and females (red circle) (n = 8). Statistical analyses were not performed due to the low number of samples at each stage and sex.