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. 2022 Nov 8;7(21):e161878. doi: 10.1172/jci.insight.161878

Figure 4. Distinct tubular uptake of apoAI versus IsoLG-apoAI.

Figure 4

(A) Cultured TECs exposed to IsoLG-apoAI showed higher expression of ABCA1 and SRBI versus apoAI. (B) TECs took up more IsoLG-apoAI versus apoAI. Scale bar: 50 μm. Knockdown of either ABCA1 or SRBI (C and D) reduced TEC uptake of apoAI but not IsoLG-apoAI. (E) In TECs exposed to apoAI, knockdown of ABCA1 or SRBI decreased expression of the other transporter. (F) In TECs exposed to IsoLG-apoAI, ABCA1 knockdown significantly increased SRBI expression. SRBI siRNA significantly increased ABCA1 expression. (G) Knockdown of both ABCA1 and SRBI reduced cellular uptake IsoLG-apoAI. In vitro, experiments were performed independently 3 times with 3 wells per treatment. Data represent mean ± SEM. Overall statistical difference determined by Kruskal-Wallis test and pairwise difference by Wilcoxon rank sum test followed by Bonferroni correction on P values. *P < 0.05, **P < 0.01, ***P < 0.001.