(A) Cultured TECs exposed to IsoLG-apoAI increased KIM-1 (HAVCR1), NLRP3, IL-1, and IL-6 gene expression versus unmodified apoAI. (B) Cultured LECs exposed to IsoLG-apoAI increased SPHK2 mRNA and reduced SPNS2 mRNA versus apoAI. (C) LECs exposed to IsoLG-apoAI increased NLRP3, IL-1, and IL-6 gene expression versus apoAI. In vitro, experiments were performed independently 3 times with 3 wells per treatment. Data represent mean ± SEM. For more than 2 groups, statistical significance was determined by Kruskal-Wallis test followed by Wilcoxon rank sum tests and Bonferroni correction on P values. *P < 0.05, **P < 0.01, ***P < 0.001.