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. 2022 Oct 10;7(19):e163795. doi: 10.1172/jci.insight.163795

Figure 4. Relationship between IFIT3 and Ro52 expression in neutrophils and PBMCs from patients with SLE and healthy controls.

Figure 4

(A and B) Neutrophils (PMN) and PBMCs from 19 consecutive patients with SLE were analyzed by immunoblotting using antibodies to IFIT3, Ro52 (D-12 mouse monoclonal antibody), histone H3 (H3), and β-actin (loading controls). Representative data from 10 patients are shown. (C and D) Correlation between the expression of IFIT3 and Ro52 in PMN and PBMCs. The expression of Ro52 and IFIT3 was quantified by densitometry from the corresponding bands in A and B, and the values were fitted to a linear regression model. (E) PBMCs and PMN from 12 healthy controls (Ctrl) and 1 patient with SLE were analyzed by immunoblotting using antibodies to IFIT3, Ro52 (D-12 mouse monoclonal antibody), and H3 (loading control). Representative data from 6 healthy controls are shown. The SLE samples were included for comparison.