Skip to main content
. 2022 Sep 22;7(18):e156549. doi: 10.1172/jci.insight.156549

Figure 5. Single-cell transcriptional evaluation of a combination treatment with 4-PBA and TUDCA on SC-β cells.

Figure 5

(A) Uniform manifold approximation and projection (UMAP) plot from unsupervised clustering of combined transcriptional data from scRNA-Seq of W024, Ctrl (green); W024, P+T (blue); W121, Ctrl (purple); and W121, P+T (red) SC-β cell populations. Lower plots are UMAP plots split by experimental conditions. (B) Violin plots detailing log-normalized gene expression of β cell genes in the same populations as A. Log fold change and P values for violin plots are available in Supplemental Table 2. (C) Violin plots detailing log-normalized gene expression of ER stress and apoptotic genes in the same populations as A. Log fold change and P values for violin plots are available in Supplemental Table 3. (D) Gene Ontology (GO) and Reactome GSEA, quantified by the normalized enrichment score (NES), for pathways upregulated in the combined population of W024 and W121 SC-β cells treated with (pink) or without (blue) P+T. NES values, P values, FDR q values, and gene set lists are available in Supplemental Table 4.