TABLE 1.
Strains and plasmids used in this study
| Strains and plasmids | Relevant characteristic | Reference or source |
|---|---|---|
| E. rhusiopathiae (serotype) | ||
| Fujisawa (1a) | Japanese official challenge strain | 20 |
| Koganei (1a) | Japanese official live vaccine strain | 20 |
| SE-9 (2a) | U.S. official bacterin strain | 6 |
| ATCC 19414T (2b) | Type strain | American Type Culture Collection |
| Marienfelde (1a) | Growth agglutination strain | 29 |
| ME-7 (1a), 422/1E1 (1b), R32E11 (2a), NF4E1 (2b), Pécs 67 (5), Goda (8), Kaparek (9), Pécs 9 (12), Pécs 3597 (15), Tanzania (16), 545 (17), MEW 22 (N) | Reference strains for serotyping | 25 |
| E. coli XL1-Blue | Host strain for plasmids | Stratagene |
| Plasmids | ||
| pBluescript II | Cloning vector | Stratagene |
| pQE32 | His6 vector | Qiagen |
| Recombinant plasmids | ||
| pA, pB | Recombinant pBluescript II of spaA/Fujisawa | This study |
| pA1.0 | Recombinant pQE32 of spaA/Fujisawa | This study |
| Recombinant E. coli XL1-Blue (pA1.0) | This study |