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AC toxin binds to sheep RBC at 0°C, but at a reduced level. Sheep RBC at 0 or 37°C were incubated with AC toxin (15 μg/ml) for the indicated time. Data represent mean fluorescence ± standard deviation from three similar experiments. Sheep RBC treated with polyclonal antibody against AC toxin and FITC-conjugated goat anti-mouse IgG served as background fluorescence. The mean fluorescence of the background is represented as the zero time point.
