| Antibodies |
| Rabbit polyclonal anti-ERK5 |
Cell Signaling Technology |
Cat#3372S; RRID: AB_330491 |
| Rabbit polyclonal anti-BRD4 |
Fortis Life Sciences |
Cat#A301-985A-M; RRID: AB_2631450 |
| Rabbit monoclonal anti-AURKA |
Cell Signaling Technology |
Cat# 14475S; RRID: AB_2665504 |
| Mouse monoclonal anti-β-Actin |
Cell Signaling Technology |
Cat#3700S; RRID: AB_2242334 |
| Chemicals, peptides, and recombinant proteins |
| XMD17-109 |
MedChemExpress |
Cat#HY-15665 |
| XMD8-92 |
MedChemExpress |
Cat#HY-14443 |
| JWG-071 |
MedChemExpress |
Cat#HY-108886 |
| BAY-885 |
MedChemExpress |
Cat#HY-112082 |
| AX15835 |
MedChemExpress |
Cat#HY-101846 |
| Bortezomib |
MedChemExpress |
Cat#HY-10227 |
| MLN4924 |
MedChemExpress |
Cat#HY-70062 |
| JQ1 |
MedChemExpress |
Cat#HY-13030 |
| Recombinant Human IL-6 (carrier-free) |
BioLegend |
Cat#570804 |
| Lipopolysaccharides from Escherichia coli O111:B4 |
Millipore Sigma |
Cat#L2630 |
| INY-05-091 |
This study |
N/A |
| INY-05-128 |
This study |
N/A |
| INY-06-061 |
This study |
N/A |
| INY-06-086 |
This study |
N/A |
| INY-06-089 |
This study |
N/A |
| Critical commercial assays |
| CellTiter Glo |
Promega |
Cat#G7570 |
| LEGEND MAX™ Human IL-6 ELISA Kit |
Biolegend |
Cat#430507 |
| LEGEND MAX™ Human IL-8 ELISA Kit |
Biolegend |
Cat#431507 |
| ERK5 Human CMGC Kinase Binding Assay, KINOMEscan (KdELECT) |
Eurofins Discovery |
Cat#87-0007-1148 |
|
scanMAX Kinase Panel, KINOMEscan |
Eurofins Discovery |
Cat#87-0001-1000 |
| Deposited data |
| INY-05-091 proteomics in MOLT4 cells |
This study |
PXD036104 |
| INY-05-128 proteomics in MOLT4 cells |
This study |
PXD036104 |
| INY-06-061 proteomics in MOLT4 cells |
This study |
PXD036105 |
| Experimental models: Cell lines |
| Human: MOLT4 |
ATCC |
Cat#CRL-1582; RRID: CVCL_0013 |
| Human: BT-474 |
ATCC |
Cat#HTB-20; RRID: CVCL_A4CL |
| Human: SNU-449 |
ATCC |
Cat#CRL-2234; RRID: CVCL_0454 |
| Human: MM.1S |
ATCC |
Cat#CRL-2974; RRID: CVCL_8792 |
| Human: Primary Umbilical Vein Endothelial Cells; Normal, Pooled (HUVEC) |
ATCC |
Cat#PCS-100-013 |
| Oligonucleotides |
| hERK5-HiBiT HDR Template |
Integrated DNA Technologies |
Cat# CD.HC9.JZMT3593 |
| hERK5-HiBiT gRNA |
Integrated DNA Technologies |
Cat# Hs.HC9.DRQY5857.AA |
| Software and algorithms |
| Graphpad Prism |
GraphPad Software, Inc. |
https://www.graphpad.com/
|
| Adobe Illustrator |
Adobe Creative Cloud |
https://www.adobe.com/creativecloud.html
|
| Columbus image storage and analysis |
PerkinElmer Informatics |
https://www.perkinelmer.com/product/image-data-storage-and-analysis-system-columbus
|
| Proteome Discoverer 2.4 |
Thermo Fisher Scientific |
https://www.thermofisher.com/order/catalog/product/OPTON-20141?SID=srch-srp-OPTON-20141
|
| R Framework |
Team RCR: A Language and Environment for Statistical Computing |
https://www.r-project.org/
|
| Python 3.10.6 |
Python Software Foundation |
https://www.python.org/
|
| Other |
| Tandem Mass Tag (TMT) Reagents |
Thermo Fisher Scientific |
Cat# A44520
|
| Bradford Reagent |
BioRad |
Cat# 500-0205 |
| cOmplete, Mini Protease Inhibitor Cocktail |
Sigma-Aldrich |
Cat#11836153001 |
| PhosSTOP Phosphatase Inhibitor Tablets |
Sigma-Aldrich |
Cat#04906837001 |
|
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